{"id":21,"date":"2025-03-14T13:38:21","date_gmt":"2025-03-14T13:38:21","guid":{"rendered":"https:\/\/www.congressi.unisi.it\/satelliteebsa\/?page_id=21"},"modified":"2025-04-29T09:04:56","modified_gmt":"2025-04-29T09:04:56","slug":"programme","status":"publish","type":"page","link":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/programme\/","title":{"rendered":"Programme"},"content":{"rendered":"\n

We offer a 4-day course comprising lectures on protein expression and purification techniques, and methods for assessing the quality of purified protein samples. Additionally, participants will have hands-on practical sessions demonstrating how to apply these experiments for protein quality control. Topics included in the course:<\/p>\n\n\n\n

Lectures<\/strong><\/p>\n\n\n\n

Day 1<\/strong>
Protein expression, purification and QC; Overview of protein expression methods; Overview of protein purification methods; Guidelines for protein quality control <\/p>\n\n\n\n

Day 2<\/strong>
PAGE, Protein concentration and optical properties of protein; Determination of protein concentration; Polyacrylamide gel electrophoresis;
Absorbance, fluorescence, circular dichroism; Hydrogen-deuterium exchange applications in protein quality control <\/p>\n\n\n\n

Day 3<\/strong>
Quality control tests; Light scattering and mass photometry; Mass spectrometry; <\/p>\n\n\n\n

Day 4<\/strong>
Storage, formulation and extended tests; Differential scanning fluorimetry; Protein storage and formulation; Protein-protein interactions<\/p>\n\n\n\n

Practical sessions<\/strong><\/p>\n\n\n\n

Session 1 : <\/strong>DLS, nanoDSF, SEC-MALS
Session 2 : <\/strong>Mass spectrometry, mass photometry
Session 3 : <\/strong>UV absorbance, fluorescence, circular dichroism<\/p>\n\n\n\n

The final programme could be found here<\/a>.<\/strong><\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

We offer a 4-day course comprising lectures on protein expression and purification techniques, and methods for assessing the quality of purified protein samples. Additionally, participants will have hands-on practical sessions demonstrating how to apply these experiments for protein quality control. Topics included in the course: Lectures Day 1Protein expression, purification and QC; Overview of protein […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"saved_in_kubio":false,"footnotes":""},"class_list":["post-21","page","type-page","status-publish","hentry"],"kubio_ai_page_context":{"short_desc":"","purpose":"general"},"_links":{"self":[{"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/pages\/21","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/comments?post=21"}],"version-history":[{"count":3,"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/pages\/21\/revisions"}],"predecessor-version":[{"id":59,"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/pages\/21\/revisions\/59"}],"wp:attachment":[{"href":"https:\/\/www.congressi.unisi.it\/icPQC-Siena2025\/wp-json\/wp\/v2\/media?parent=21"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}