We offer a 4-day course comprising lectures on protein expression and purification techniques, and methods for assessing the quality of purified protein samples. Additionally, participants will have hands-on practical sessions demonstrating how to apply these experiments for protein quality control. Topics included in the course:
Lectures
Day 1
Protein expression, purification and QC; Overview of protein expression methods; Overview of protein purification methods; Guidelines for protein quality control
Day 2
PAGE, Protein concentration and optical properties of protein; Determination of protein concentration; Polyacrylamide gel electrophoresis;
Absorbance, fluorescence, circular dichroism; Hydrogen-deuterium exchange applications in protein quality control
Day 3
Quality control tests; Light scattering and mass photometry; Mass spectrometry;
Day 4
Storage, formulation and extended tests; Differential scanning fluorimetry; Protein storage and formulation; Protein-protein interactions
Practical sessions
Session 1 : DLS, nanoDSF, SEC-MALS
Session 2 : Mass spectrometry, mass photometry
Session 3 : UV absorbance, fluorescence, circular dichroism
The final programme could be found here.
